Eosin methylene blue agar (EMB) is a selective and differential medium used to isolate fecal coliforms. Eosin Y and methylene blue are pH indicator dyes which combine to form a dark purple precipitate at low pH; they also serve to inhibit the growth of most Gram positive organisms
Methylene blue‎: ‎0.065 g
Agar‎: ‎13.5 g
Eosin Y‎: ‎0.4 g
Lactose‎: ‎5 g