Deionised Water HPLC 2.5L

Deionised Water HPLC 2.5L

In an HPLC (High-Performance Liquid Chromatography) lab, deionized (DI) water is primarily used to prepare chromatography solvents, buffers, and standards because its purity prevents contamination and interference with the separation process and detector signals. It’s also used for cleaning lab equipment like glassware to avoid introducing impurities or mineral deposits that could affect subsequent analyses.

Deionised Water HPLC 2.5L

Specific Uses of Deionized Water in an HPLC Lab
Solvent & Mobile Phase Preparation:
DI water is a key component for preparing HPLC mobile phases, ensuring that no ionic or dissolved impurities interfere with the sensitive analytical separation of samples.
Buffer and Reagent Preparation:
It is used to accurately create buffers and other reagents used in HPLC analysis, ensuring consistency and reliability in experiments.
Sample Dilution:
DI water is used to dilute samples for analysis, maintaining the purity and integrity of the sample and preventing unwanted chemical interactions.
Cleaning and Rinsing:
Rinsing laboratory equipment with DI water removes residual contaminants from previous experiments, preventing cross-contamination that can compromise the accuracy of future tests.
Why Purity is Essential for HPLC
Prevents Baseline Noise and Ghost Peaks:
Trace impurities in water can cause noise or spurious peaks in the chromatogram, making it difficult to interpret results.
Maintains Equipment Integrity:
The absence of ions and minerals prevents the formation of mineral deposits in HPLC systems, reducing corrosion and extending the lifespan of sensitive instruments.
Ensures Accurate Results:
By providing a pure, contaminant-free base, DI water ensures that experimental results are solely due to the sample being analyze
HPLC bottled water is specially treated to be most free of organics.

Deionised Water HPLC 2.5L

• There might be traces of ions.

Deionised Water HPLC 2,5L

Deionised Water HPLC 2.5L