3M MRS Agar 500g
Discover the benefits of MRS Agar 500g from Masiye Labs for reliable microbiological analysis and research applications.
MRS Agar is a medium for the enumeration of mesophilic lactic acid bacteria according to ISO 15214:1998, and is not intended for use in the diagnosis of disease or other conditions in humans.
MRS Agar was originally developed in 1960 by de Man, Rogosa & Sharpe for the cultivation and enumeration of Lactobacillus spp.
Nutrition is provided by enzymatic digest of casein, glucose, meat & yeast extracts whilst polyoxyethylenesorbitan monooleate, magnesium and manganese sulphates act as growth stimulants.
Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli whilst inhibiting a number of other organism groups.
Occasionally, sterilization of this medium at 121°C can cause the pH to fall outside of the specified pH limits of 5.7 +/- 0.1.
In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.
